Evaluation of the precision of gene expression pattern registration to the nuclear-stain and the tubulin templates. (A, B) Absolute deviation of center positions of the corresponding cells (that is, distances of the individual cells to their average coordinate) for 48 hpf (A) and 72 hpf (B) larvae for the indicated cells for the nuclear-stain and the tubulin templates. The cells and their identifiers are shown in Figure 3. The graphs represent min-max values, (+) indicates mean values. P values of a paired t-test are shown: *P <0.05, **P <0.01. hpf, hours post fertilization.