Experimental verification of coexpression for a gene pair showing colocalization in the atlas. (A-D) Colocalization of the average signals for r-opsin-1 (cyan) and TrpC (magenta) in a 72 hpf larva, as determined by image registration to the nuclear-stain template. (E-H) Coexpression of r-opsin-1 (cyan) and TrpC (magenta) in a 72 hpf larva, as determined by double in situ hybridization. (D, H) are close up images of the boxed areas shown in C and G. H only shows a single plane. Scale bar (A-C, E-G) 30 μm, (D, H) 10 μm. DLE, dorsal larval eye; hpf, hours post fertilization; VLE, ventral larval eye.