Effect of sample size on the absolute deviation of cell center positions. We determined the average cell body coordinates of the indicated cells from ten scans each. We sampled random subsets of one to nine (1000 each) from the complete set of each cell. The absolute deviation of cell center positions of the different subsets relative to the complete set average are shown for 48 hpf (A) and 72 hpf (B) larvae. The cells and their identifiers are shown in Figure 3. We chose the two best and the two worst registered cells for both stages. The graphs represent min-max values, (+) indicates mean values. hpf, hours post fertilization.