Maximum parsimony character state reconstructions of nucleotide changes within the C-terminus of graminoid Poales LOFSEP proteins. Reconstructed evolution of the codon immediately after the ZMM3 domain estimated a change from glutamine to stop codon at the base of the OSM5 lineage (left hand side). Multiple codons per branch separated by vertical lines represent equally parsimonious reconstructed amino acids. Aligned ZMM3 and OSMADS1 motifs among LOFSEP genes (right hand side). Colored boxes = translated amino acids, Uncolored boxes = non-coding nucleotides. Beige box = Aspartic acid, Black box = Stop, Pale Blue box = Alanine (A), Purple box = Histidine (H), Red box = Glutamine (Q). Green = LEAFY HULL STERILE1 (LHS1) clade, Blue branches = Oryza sativa MADS5 (OSM5) clade.