Scanning Electron Microscopy (SEM) analysis and mRNA expression profiles of OSM5 co-orthologs in Avena sativa, Chasmanthium latifolium, Hordeum vulgare, Pennisetum glaucum , and Sorghum bicolor inflorescences. Scanning electron microscopy (SEM) images of inflorescence development in Avena sativa (A), Chasmanthium latifolium (E), and Hordeum vulgare (I), mRNA in situ hybridization expression analyses of OSM5 co-orthologs in developing inflorescences of A. sativa (B-C), C. latifolium (F-G), H. vulgare (J-K), P. glaucum (M) and S. bicolor (O) using antisense digoxygenin-labeled RNA probes, and cartoons summarizing OSM5 expression in the different taxa (D, H, L, N and P). Developing A. sativa spikelets with AsOSM5 expression in lemmas and paleas (B-D). Developing C. latifolium spikelets with ClOSM5 expression in the spikelet meristem, lemma and palea of upper florets and no expression in the lower florets (F-H). Developing H. vulgare spikelets with HvOSM5 expression in lemmas and lodicules (J-L). P. glaucum spikelets with PgOSM5 expression in carpels, stamens and lodicules of the upper floret and stamens and lodicules of the lower spikelet (M-N). Young S. bicolor spikelet with SbOSM5 expression in the palea and lemma of the upper floret and sterile lemma of the lower floret (O-P). Abbreviations: c = carpels, l = lemma, lo = lodicule, p = palea, s = stamen, sm = spikelet meristem. Bars, 100 μm.