Maximum likelihood character reconstructions of graminoid poales LOFSEP gene expression in inflorescences and spikelets. (A) Expression evolution in different regions of the inflorescence and spikelet. Black = Expression restricted to the lower floret within the spikelet, Grey = Expression restricted to the upper floret in the spikelet, White = Expression present in all flowers of the inflorescence. (B) Evolution of floral maturation. Black = basipetally maturing spikelets, Grey = Flowers borne singly or spikelets comprised of a single floret, White = acropetally maturing spikelets. Green = LEAFY HULL STERILE1 (LHS1) clade, Blue branches = Oryza sativa MADS5 (OSM5) clade.