Table 4 Trinity assembly compared to digital normalization/Velvet/Oases
| Â | Trinity assembly | Digital normalization, Oases-Velvet |
|---|---|---|
Total fragments | 87,209,130 (100.00%) | 87,209,130 (100.00%) |
Aligned concordantly 0 times | 23,101,349 (26.49%) | 35,552,373 (40.77%) |
Aligned concordantly 1 time | 28,325,506 (32.48%) | 30,552,963 (35.03%) |
Of reads aligning concordantly or discordantly 0 times: | Â | Â |
  Aligned discordantly 1 time | 1,826,073 (7.90%) | 7,539,150 (21.21%) |
  Total mates | 42,550,552 | 56,026,446 |
  Aligned 0 times | 24,594,154 (57.80%) | 31,100,316 (55.51%) |
  Aligned exactly 1 time | 4,660,328 (10.95%) | 11,909,789 (21.26%) |
  Aligned >1 times | 13,296,070 (31.25%) | 13,016,341 (23.23%) |
Overall alignment rate | 85.90% | 82.17% |