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Table 4 Trinity assembly compared to digital normalization/Velvet/Oases

From: A quantitative reference transcriptome for Nematostella vectensis earlyembryonic development: a pipeline for de novo assembly in emergingmodel systems

  Trinity assembly Digital normalization, Oases-Velvet
Total fragments 87,209,130 (100.00%) 87,209,130 (100.00%)
Aligned concordantly 0 times 23,101,349 (26.49%) 35,552,373 (40.77%)
Aligned concordantly 1 time 28,325,506 (32.48%) 30,552,963 (35.03%)
Of reads aligning concordantly or discordantly 0 times:   
  Aligned discordantly 1 time 1,826,073 (7.90%) 7,539,150 (21.21%)
  Total mates 42,550,552 56,026,446
  Aligned 0 times 24,594,154 (57.80%) 31,100,316 (55.51%)
  Aligned exactly 1 time 4,660,328 (10.95%) 11,909,789 (21.26%)
  Aligned >1 times 13,296,070 (31.25%) 13,016,341 (23.23%)
Overall alignment rate 85.90% 82.17%