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Figure 1 | EvoDevo

Figure 1

From: Expression of germline markers in three species of amphioxus supports a preformation mechanism of germ cell development in cephalochordates

Figure 1

Protein domain structure and phylogenetic analysis of Branchiostoma floridae Piwi/Ago family sequences. (A) Schematic depiction of the composition of major domains in B. floridae Piwi and Argonaute (Ago) proteins (with abbreviation Bf), and related proteins from Drosophila melanogaster (Dm) and human (Hs). Conserved protein domain information is provided in Additional file 2: Table S2. (B) Phylogenetic tree of Piwi/Ago family proteins constructed using the neighbor-joining method. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1,000 replicates) is shown next to each branch. When the supporting percentage of a particular node is less than 60, the value is not shown on the node. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the p-distance method and are presented as the number of amino acid differences per site. The analysis involved 35 amino acid sequences; their NCBI accession numbers are listed in Additional file 3. Species abbreviations: Bf, Branchiostoma floridae (Florida amphioxus); Bt, Bos taurus (cattle); Ch, Clytia hemisphaerica (hydra); Ci, Ciona intestinalis (tunicate); Dm, Drosophila melanogaster (fruit fly); Dr, Danio rerio (zebrafish); Ef, Ephydatia fluviatilis (sponge); Hm, Hydra magnipapillata (hydra); Hs, Homo sapiens (human); Mm, Mus musculus (mouse); Nv, Nematostella vectensis (sea anemone); Pc, Podocoryne carnea (jellyfish); Pt, Pan troglodytes (chimpanzee); Sp, Strongylocentrotus purpuratus (purple sea urchin).

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