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Figure 1 | EvoDevo

Figure 1

From: Developmental coupling of larval and adult stages in a complex life cycle: insights from limb regeneration in the flour beetle, Tribolium castaneum

Figure 1

Effects of patterning gene knockdown on embryonic appendage development. Eggs and newly hatched larvae from female beetles injected with of 2 μg/μL ampicillin-resistance (ampr) (A-C) , dachshund (dac) (D-F) and abrupt (ab) (G-I) double-stranded (ds)RNA were collected and processed to compare the phenotypes of resulting antennae and legs. Newly hatched ampr dsRNA-treated larvae were used as a control. The antennae (B,E,H) and legs (C,F,I) were visualized under fluorescence microscopy and are marked with arrowheads. Right panel shows a color-level inversion image with the leg segments highlighted in yellow. (J) Lateral view of the abdomen of a newly hatched larva obtained from female adults injected with ampr dsRNA. (K) Lateral view of the abdomen of the late embryo obtained from female adults injected with ab dsRNA. Scale bars represent 0.1 mm.

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ISSN: 2041-9139