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Figure 2 | EvoDevo

Figure 2

From: Developmental coupling of larval and adult stages in a complex life cycle: insights from limb regeneration in the flour beetle, Tribolium castaneum

Figure 2

The effect of patterning gene knockdown on Tribolium larval leg regeneration one or two molts after leg ablation . (A,C,E,G,I,K) Regenerating larval legs after one molt in larvae injected with ampicillin-resistance (ampr) double-stranded (ds)RNA (A), dachshund (dac) dsRNA (C), Distal-less (Dll) dsRNA (E), abrupt (ab) dsRNA (G), spineless (ss) dsRNA (2 μg/μl) (I) and ss dsRNA (4 μg/μl) (K). (B,D,F,H,J,L) Regenerating larval legs after two molts in larvae injected with ampr dsRNA (B), dac dsRNA (D), Dll dsRNA (F), ab dsRNA (H), ss dsRNA (2 μg/μl) (J) and ss dsRNA (4 μg/μl) (L). Arrowheads indicate the bases of the ablated legs. Middle panel shows the close-up image of the hindleg. Right panel shows a color-level inversion image with the regenerating leg segments highlighted in yellow. Cx, coxa; tr, trochanter; f, femur; tt, tibiotarsus. Scale bars correspond to 0.1 mm.

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ISSN: 2041-9139