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Figure 3 | EvoDevo

Figure 3

From: Developmental coupling of larval and adult stages in a complex life cycle: insights from limb regeneration in the flour beetle, Tribolium castaneum

Figure 3

Regenerated legs of larvae after three molts post gene knockdown and leg ablation. (A-C) Regenerating larval legs injected with ampicillin-resistance (ampr) double-stranded (ds)RNA (A), dachshund (dac) dsRNA (B) and spineless (ss) dsRNA (4 μg/μl) (C). The mid- and hindlegs (marked with arrowheads) were removed. A close-up of the hindleg is shown in the center, and right panels show a color-level inversion image with the regenerated leg segments highlighted in yellow. Fourth-instar larvae were injected on day 0, and legs were removed on day 2.

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ISSN: 2041-9139