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Figure 4 | EvoDevo

Figure 4

From: Developmental coupling of larval and adult stages in a complex life cycle: insights from limb regeneration in the flour beetle, Tribolium castaneum

Figure 4

The effects of appendage patterning gene knockdowns on adult leg regeneration in Tribolium . (A, B) Adult leg phenotype of larvae injected with 2 μg/μL ampicillin-resistance (ampr) double-stranded (ds)RNA. Adults formed from pupae that experienced one molt (A) or two molts (B) after dsRNA injection. Close-up of the unablated (middle) and ablated (right) mid- (top) and hindlegs (bottom) are shown. (C,D) Adults that formed after larvae were injected with 2 μg/μL dachshund (dac) dsRNA and underwent one molt (C) and two molts (D) before pupation. Close-up of the unablated (middle) and ablated (right) mid- (top) and hindlegs (bottom) are shown. Arrowheads indicate the segment on the unablated leg that is missing in the ablated leg. (E,F) Adults that formed after larvae were injected with 2 μg/μL abrupt (ab) dsRNA and underwent two molts before pupation. Close-up of the unablated (middle) and ablated (right) mid- (top) and hindlegs (bottom) are shown. (E) Arrows indicate the site where the legs should have regenerated. (F) Arrow indicates the claw-like structure that developed at the base of the leg. (G,H) Adults that formed after larvae were injected with 4 μg/μL ss dsRNA and underwent one molt (G) and two molts (H) before pupation. Close-up of the unablated (middle) and ablated (right) mid- (top) and hindlegs (bottom) are shown. (I) Adults that formed after larvae were injected with 2 μg/μL Distal-less (Dll) dsRNA and underwent three molts before pupation. Arrows indicate the sites where the legs should have regenerated.

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