Developmental coupling of larval and adult stages in a complex life cycle: insights from limb regeneration in the flour beetle, Tribolium castaneum

Table 1 Primers used for ab, dac, Dll and ss dsRNA synthesis and knockdown verification

Gene	Application	Forward primer (5′->3′)	Reverse primer (5′->3′)
ab	RNAi	ACTCACAAAGGAGAAGGGAAA	CGTTGGTATTGAAAGGATGG
ab	Knockdown verification	GCCTGTGATGGATGTTCGT	GTCTTGGGTCTGTCGCTCT
ss	RNAi	ATTACTCAAAACTGGCGCTTC	TGTTGTGTTAGTGGGAGGAGTT
ss	Knockdown verification	GGAGATACCGCAGAAGGAAG	TTGTAGTCAGGGGCGATGT
dac	RNAi	CAGCATCGCATCTTCAAC	CTCCTCCCTCAGCCTTTCT
dac	Knockdown verification	ACTGCACTACGGCCAGTTC	TCGTCCATGTCTTGATCCTT
Dll	RNAi	GGATAACAAACCCTTCACGAC	GCCTCTCCAACGATAAACAC
	Knockdown verification	GGTGTGTTCGTAGTGCTTCC	CGCCTTCATCATCTTCTTGT

RNAi, RNA interference; ab, abrupt; ss, spineless; dac, dachshund; Dll, Distal-less.

ISSN: 2041-9139