Developmental coupling of larval and adult stages in a complex life cycle: insights from limb regeneration in the flour beetle, Tribolium castaneum

Table 4 Summary of effects of patterning gene knockdowns on larval leg regeneration in Tribolium

			Larvae after 2 molts			Pupae after 1 molt		Pupae after 2 molts
dsRNA injected	Concentration	Total number	Number with regenerated legs	Number with no regenerated legs	Died before pupation after 2 larval molts	Number with leg regeneration	Number with no leg regeneration	Number with leg regeneration	Number with no leg regeneration
amp ^r	2 μg/μL	23	10	0	3	13	0	7	0
dac	2μg/μL	18	16*	0	4	2	0	12*	0
Dll	2μg/μL	18	0	18	16	0	0	0	2
ab	2 μg/μL	28	0	19	13	0	9	0	6
ss	4 μg/μL	18	11	0	3	7	0	8	0
	2 μg/μL	10	7	0	1	3	0	6	0

The fifth- or sixth-instar larvae were injected with amp^r, dac, Dll, ab and ss dsRNA, and their legs were ablated. amp^r dsRNA injections served as controls. *Regeneration in the dac dsRNA-injected insects was different from that in the control amp^r dsRNA-injected insects. amp^r, ampicillin-resistance; dac, dachshund; Dll, Distal-less; ab, abrupt; ss, spineless.

ISSN: 2041-9139