Developmental coupling of larval and adult stages in a complex life cycle: insights from limb regeneration in the flour beetle, Tribolium castaneum

Table 5 Summary of the effects following knockdown of patterning genes on larval antennal regeneration in Tribolium

			Larvae after 2 molts			Pupae after 1 molt		Pupae after 2 molts
dsRNA injected	Concentration	Total number	Number with regenerated antennae	Number with no regenerated antennae	Died before pupation after 2 larval molts	Number with antennal regeneration	Number with no antennal regeneration	Number with antennal regeneration	Number with no antennal regeneration
amp ^r	2 μg/μL	14	10	0	0	4	0	10	0
dac	2 μg/μL	27	14	0	2	10	3	12	0
Dll	2 μg/μL	10	0	7	7	0	3	0	0
ab	2 μg/μL	14	4	7	11	2	1	0	0
ab	0.5 μg/μL	9	2	7	8	0	0	1	0
ss	2 μg/μL	22	21*	0	9	0	1	12*	0

The left antennae of fifth- and sixth-instar larvae treated with abrupt (ab), dachshund (dac), Distal-less (Dll) and spineless (ss) double-stranded (ds)RNA at various concentrations were ablated. The numbers of Tribolium treated with the ampicillin-resistance (amp^r) dsRNA are also included as a control. *For ss, the pattern of regeneration was different from the control amp^r dsRNA-injected regeneration; the regenerating antennae transformed into a leg.

ISSN: 2041-9139