Figure 1

Al-patched ( Al-ptc ) expression. A-D´, G, I, and J-O are oriented with the anterior pole towards the left of the page. (A) Ventral Al-ptc expression in an early embryo with Al-ptc in the Pp and L1-L2. (B) Ventral-lateral view of the same embryo showing double Al-ptc in the Ch and the GZ. (C) Lateral view of the embryo in A and B. (C´) DAPI image of the embryo in C. (D) Anterior view of the same embryo showing Al-ptc in the Oc. (D´) DAPI image of the embryo in D. (E) Anterior view of the same embryo, showing Al-ptc in the Oc. (F) The posterior end of the same embryo showing Al-ptc in the Pr and the GZ. (G) Lateral view of a late embryo showing stripes of Al-ptc in the prosoma with a stripe of expression in the PL3. (H) Posterior view of the embryo in G. (H´) DAPI image of the embryo in H. (I) Ventral view of an embryo showing Al-ptc in O1. (J) Ventral-lateral view of an embryo showing Al-ptc in O1. (K) Lateral view of a later-stage embryo showing Al-ptc in O1 and in the L4. (L) Ventral view of a later-stage embryo showing the split stripe of Al-ptc in O1. (M) Ventral-lateral view of a later embryo showing the split stripe of Al-ptc in O1 (arrowheads), L4 and O2. (N) Lateral view of the opisthosoma of a late embryo showing Al-ptc in O1 and O2. (O) Ventral view of the embryo in N. Scale bars in C´ and D´ are 50 μm. Ch, chelicerae; DAPI, 4′,6-diamidino-2-phenylindole; GZ, growth zone; L1-L3, first through third walking legs; L4, fourth walking leg anlagen; O1-O2, first and second opisthosomal segment; Oc, ocular segment; PL3, posterior portion of the L3 segment; Pp, pedipalps; Pr, proctodaeum; VS, ventral sulcus.