Figure 7

Ciliary band and antibody staining in Maritigrella larvae. (A) through (C) Schematic drawings of 1-day-old larvae (from left to right: ventral view, lateral view and posterior view, respectively) featuring the ciliary band cells in purple. Numbers are related to rows of ciliary band cells. (D) Posterior view of fixed larva with stained ciliary band cells. (E) Larva after a 20-h bromodeoxyuridine (BrdU) pulse: S-phase-labelled cells are shown in green using an antibody against BrdU, and mitoses are shown in red using an antibody against phosphorylated histone H3. Dashed outline: ventral is left and anterior is up. (F) through (G) Larval brain seen from the anterodorsal side. Staining with antibodies against the neuropeptides AVRLIRLamide (F) and GVWSNDPWamide (G). Asterisks demarcate the position of the cerebral eyes. (H) Ciliary band cells visualised by differential interference contrast imaging. Purple in (D) and (H) is unspecific staining. Scale bars are 50 μm in (D) and (E) and 10 μm in (G) and (H). Scale bar in (F) identical to that in (G).