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Figure 3 | EvoDevo

Figure 3

From: Nuclearization of β-catenin in ectodermal precursors confers organizer-like ability to induce endomesoderm and pattern a pluteus larva

Figure 3

Results of cell transplantation experiments at 3 days post fertilization. (A) Uninjected control. (B) Animal half (AH) control. (C) Bright field image of a 3-day chimera formed from a labeled mesomere pair injected with 0.5 pg/pL actβ-cat mRNA recombined with an uninjected AH. Note that features such the spicules, a tripartite gut, and pigment cells are all present. Larvae appear to be normal. (C’) Fluorescent image shows that the actβ-cat mRNA-injected mesomeres gave rise to mesenchyme-like cells and pigment cells (arrows in C, C’, D, and D’ indicate pigment cells). (D, D’) Different focal plane in the same embryo, showing that cells in the gut are not derived from the actβ-cat mRNA-injected mesomeres. Arrowheads in C, C’, D, and D’ indicate the induced, unlabeled gut.

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ISSN: 2041-9139