Nuclearization of β-catenin in ectodermal precursors confers organizer-like ability to induce endomesoderm and pattern a pluteus larva

Table 1 Cell transplantation data for 23 to 27 hours post fertilization Lytechinus variegatus embryos

Embryo type	Animalized	Complete or partial gut	Mes-like cells	Spicules^***	Pigment cells	Swimming
Uninjected AH	92% (33/36)	8% (3/36)	11% (4/36)	0% (0/25)	6% (2/36)	14% (5/36)
Uninjected micro pair + uninjected AH	0% (0/36)^**	63% (10/16)^**	100% (16/16)^**	100% (16/16)^**	25% (4/16)^**	91% (10/11)^**
Uninjected meso pair + uninjected AH	79% (11/14) (3 of these had a few loose cells)	14% (2/14)	29% (4/14)^* 7% (1/14) solid	0% (0/11)	0% (0/14)	7% (1/14)
0.5 Actβ-cat meso pair + uninjected AH	18% (9/50)^**	54% (27/50)^**	82% (41/50)^**	82% (41/50)^**	78% (39/50)^**	82% (41/50)^**
1.0 Actβ-cat meso pair + uninjected AH	17% (4/23)^**	30% (7/23)^**	83% (19/23)^**	59% (13/22)^**	52% (12/33)^**	70% (16/23)^**

Mes-like cells indicated the presence of individual cells in the blastocoel. ^*Chi-square test indicates significant difference of 0.05>P>0.01 when compared to uninjected animal half (AH). ^**Chi-square test indicates significant difference of 0.01>P>0.001 when compared to the uninjected AH. ^***Because the expected value was 0, this produces a numerator of 0 in the Chi-square test, so a larger expected value of 1 was substituted to perform the test for significance.

ISSN: 2041-9139