Figure 9

Structure and mitoses in advanced walking leg neuromere 1 of Pseudopallene sp. (late ES 6 – ES 10) – part II. Tubulin- and PH3-labelling with nuclear counterstain. Two transverse optical sections through the ganglion anlagen of late ES 6 (A,A’), ES 8 (B,B’) and pre-hatching ES 10 (C,C’), respectively. Dashed vertical line at the bottom of each image indicates the VMR. Small white lines connect newly forming nuclei during telophase. With ongoing development, the large apical NSCs (asterisks) are underlain by a growing number of GCs (including INPs). Stars label smaller flask-shaped cells that are in the process of immigration. Solid arrowheads mark sub-apical INPs in division. Open arrowheads mark developing neuropil and axonal pathways at the basal side of the ganglion anlagen. Arrows indicate flattened median cells of the VMR (presumably glial cells). The upper solid arrowhead in C marks one of the few basal NPs in division.