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Figure 3 | EvoDevo

Figure 3

From: Myogenesis in the sea urchin embryo: the molecular fingerprint of the myoblast precursors

Figure 3

Co-expression analysis of putative sea urchin myogenic factors and MHC by double confocal fluoresecent in situ hybridization (FISH). Relative spatial domain of expression of FoxF (A) , FoxC (B) , FoxY (C) , FoxL1 (D) , Six1/2 (E) , SoxE (F) , MaF (G) , Myocardin (H) , MyoR2 (I) , Tbx6 (J) , Mef2 (K) , and Twist (L) (green) with respect to MHC (red) by double FISH in the late gastrula stage, 48 to 50 h. Every picture is a full projection of merged confocal stacks. Aside each picture are separately placed single focal planes of each channel plus a merged picture of an enlarged detail at the tip of the archenteron, to clarify any misleading issue of co-expression domains. In E, a single brightfield slice is also superimposed to the red channel. Yellow circles indicated by yellow arrowheads show co-expressing cells, and the white ones point to absence of co-expression. The white arrows indicate other domains of expression. All the embryos are viewed along the animal top/vegetal down (A/V) axis from the oral or aboral surface, excluding the one reported in L which is shown in a lateral view along the A/V axis with the oral side on the right. Nuclei are stained blue with 4',6-diamidino-2-phenylindole (DAPI).

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