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Figure 7 | EvoDevo

Figure 7

From: Myogenesis in the sea urchin embryo: the molecular fingerprint of the myoblast precursors

Figure 7

Co-expression analysis of sea urchin putative myogenic factors and non-skeletogenic mesoderm (NSM) and small micromere (SM) molecular markers. (A-I) Expression of FoxC, FoxY, Ese, Gcm, Six1/2, SoxE, Tbx6 and Nanos was localized by double confocal fluorescent in situ hybridization at the very early gastrula stage, 30 to 32 h. Every picture is a full projection of merged confocal stacks. Yellow circles indicated by yellow arrowheads show co-expressing cells, and the white ones show absence of co-expression. For single-channel projections of the images reported in C, F and I see Additional file 11: Figure S9. All the embryos are viewed in a vegetal view with the exception of A, D, E, G and H that are seen in lateral view along the animal top/vegetal down axis. Nuclei are labeled blue with 4',6-diamidino-2-phenylindole (DAPI). In panels J and K a schematic representation is shown of a regulatory stage map of NSM of a 30-h sea urchin embryo orientated along the oral right/aboral left (O/Ab) axis in both lateral (J) and vegetal views (K). The different mesodermal cell types identified by specific regulatory signatures at the vegetal plate are shown in different colors: Mp, putative myoblast precursors, green with orange and red horizontal lines; BCp, blastocoelar cell precursors, yellow; PCp, pigment cell precursors, blue; SMd + Y, small micromere derivatives (SMd) plus FoxY expressing NSM cells (Y), green with an orange horizontal line. A legend indicates the names of the genes expressed in each region. For the sake of simplicity, primary mesenchyme cells are not shown.

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