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Figure 1 | EvoDevo

Figure 1

From: Identification of a putative germ plasm in the amphipod Parhyale hawaiensis

Figure 1

Germ line-associated RNAs localize to a distinct cortical cytoplasmic region in one-cell embryos. (A–C) vasa transcript localization. (A) In one-cell embryos, vasa is localized to a distinct cytoplasmic region at the cortex of the embryo (arrowhead) and to the cytoplasm surrounding the nucleus. (B) In two-cell and (C) eight-cell embryos, vasa transcripts are predominantly localized to the cytoplasm surrounding the nuclei and are no longer seen in a distinct cortical domain. (D–F) gcl transcript localization. (D) Like vasa, gcl is localized to a distinct cortical cytoplasmic domain in one-cell embryos (arrowhead). (E,F) In all stages subsequent to the first cell division, gcl transcripts are only detected surrounding the nuclei. (G-I) orb transcript localization. (G) Similar to vasa and gcl, orb transcripts are localized to a distinct cytoplasmic region at the cortex of one-cell embryos (arrowhead) as well as to the cytoplasm surrounding the nucleus. (H,I) Following cell division, orb transcripts are predominantly localized to the cytoplasm surrounding the nuclei. Scale bar: 200 μm (all panels).

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