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Figure 3 | EvoDevo

Figure 3

From: Identification of a putative germ plasm in the amphipod Parhyale hawaiensis

Figure 3

The cell division delay that is stereotypical of the g blastomere is lost in RCB-ablated embryos. (A-B) Still images from time-lapse recordings just subsequent to the fourth mitotic division. (A) Control embryo in which cytoplasm was removed from a spot approximately 100 μm from the RCB. Division of the g blastomere (red) is delayed with respect to the other micromeres (purple), which have already divided. (B) RCB-ablated embryo in which the g micromere (green) divided with the other micromeres (blue). (C) Scatter plot showing time points of the fourth mitotic division for individual micromeres. For control embryos, the g micromere is marked in red and the ml, mr and en micromeres are labeled with purple. For RCB-ablated embryos, micromeres with morphology consistent with that of g are marked with green and the ml, mr and en micromeres are labeled with blue. For RCB-ablated embryos in which g was not unambiguously identifiable based on morphology, all micromeres are marked in blue. In control embryos, division of g is generally delayed with respect to the other micromeres, while in RCB-ablated embryos, this delay in division is not observed. Scale bar: 200 μm (A and B) en, endoderm; g, germ line; ml, left trunk mesoderm; mr, right trunk mesoderm; RCB, RNA-containing body.

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