Figure 4
From: Identification of a putative germ plasm in the amphipod Parhyale hawaiensis
vasa-positive cells are absent in RCB-ablated embryos. (A,B) Wild-type control embryos. (A) vasa is expressed in primordial germ cells in a wild-type embryo at the early germ disc stage (arrow). (B) False-colored vasa in situ hybridization (pink) with Hoechst to label the nuclei (white). (C,D) RCB-ablated embryos. (C) vasa expression is not seen in a germ disc stage embryo, although Hoechst staining (D) shows that a germ disc has formed. Scale bar: 200 μm (all panels). RCB, RNA-containing body.