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Figure 5 | EvoDevo

Figure 5

From: Identification of a putative germ plasm in the amphipod Parhyale hawaiensis

Figure 5

RCB-ablated embryos do not form a rosette and display a defect in anterior-posterior polarity. (A–D) Still images from time-lapse movies of embryos developing from eight cells to early germ disc. (A) A control embryo in which cytoplasm was removed from a spot approximately 100 μm from the RCB. At stage 7, descendants of the Mav macromere and ml, mr and g micromeres form a rosette structure (arrow). (B) In stage 7 RCB-ablated embryos, a rosette structure does not form. (C) During stage 8, control embryos form a germ disc at the anterior end of the embryo (arrowhead). (D) Stage 8 RCB-ablated embryos form a germ disc, but it does not move to the anterior of the embryo; instead the germ disc remains at the center. Scale bar: 100 μm (all panels). RCB, RNA-containing body.

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