Cell proliferation in E. multilocularis metacestodes. (A) Detection of EdU incorporation and of H3S10-P in paraplast sections of different stages of development (some unspecific staining is seen in suckers and rostellum of protoscoleces for H3S10-P staining). (B) Whole-mount detection of EdU incorporation in a larval vesicle. The asterisk indicates an early brood capsule bud, the open arrowhead a brood capsule with a protoscolex bud, and the filled arrowhead an invaginated protoscolex. Notice also the dispersed EdU + cells in the germinative layer. (C) Whole-mount EdU detection (red) and FMRFamide immunofluorescence (green) during early protoscolex development. Most EdU + cells are located behind the main transverse commissure. (D) Whole-mount detection of EdU incorporation (red) during late protoscolex development. The arrowhead indicates the accumulation of EdU + cells at the base of the sucker. The inset shows EdU + cells in the base of a developing sucker as seen in a paraplast section. Abbreviations: bc, brood capsule; gl, germinal layer; ll, laminated layer; mlnc, main lateral nerve cord; mtc, main transverse commissure; r, rostellum or rostellum; s, sucker; st, stalk. Bars represent 30 μm except for B, 100 μm.