WMISH detection of em-h2b . (A) General view of the germinal layer. (B) Detail of the germinal layer; showing the germinative cell morphology of the positive cells. (C) Early brood capsule bud. (D) Protoscolex bud. (E) Late protoscolex development. The arrowhead indicates the accumulation of positive cells at the base of the sucker. (F) Co-localization of em-h2b (dark precipitate) and EdU detection (red) after a 50 μM, five hour pulse. The inset shows an example in which the em-h2b signal was inverted and pseudo-colored in green to facilitate the visualization of the co-labeling. Abbreviations are as in Figure 2. Bars represent 25 μm.