Expression of em-muc-1 . (A) Semi-quantitative RT-PCR with serial ten-fold dilutions of cDNA from protoscoleces (PS) and germinal layer (GL). Controls without reverse transcriptase (RT) and without template (H20) are included. (B-D) WMISH of em-muc-1. (B) General view of the germinal layer. (C) Double detection of WMISH (green) and EdU incorporation after a five hour, 50 μM EdU pulse (red); note the lack of EdU labeling among the em-muc-1+ cells. (D1 and D2) Section of metacestode processed for WMISH, showing the lack of expression in the developing protoscoleces, strong expression in the germinal layer, and expression in the brood capsule wall. Abbreviations are as in Figure 2. Bars represent 20 μm except for B, 10 μm.