Phylogenetic analyses of insect Dorsal-like proteins and expression of up-stream factors. (A) No expression of Am-pipe mRNA is found in honeybee ovarioles. (B) Am-pipe mRNA is first detected throughout the posterior third of the stage 6 embryo (C) Am-cact mRNA is enriched to the dorsal side of the oocyte (stage 4 oocyte shown). (D) By the end of oogenesis (stage 9), Am-cact mRNA is enriched to the anterior pole of the oocyte. (E) Prior to gastrulation, Am-cact mRNA expression remains restricted to an anterior domain in the cellularised embryo. (F) Consensus Bayesian analysis tree for insect Dorsal-like proteins. Honeybee Dorsal-like proteins are indicated by shading. Numbers shown at the nodes are posterior probability values. (G) Am-GB19066 RNA is detected in the nurse cells and throughout the oocyte (mid-stage oocyte shown). (H) Am-GB19066 RNA is found throughout stage 4 embryos. (I) Expression of Am-GB18032 RNA is only detected in the posterior nurse cells during mid-oogenesis (asterisks). Abbreviations: Apis mellifera (Am), Drosophila melanogaster (Dm), Tribolium castaneum (Tc), Bombus impatiens (Bimp), Nasonia vitripennis (Nv), Atta cephalotes (Acep), Acromyrmex echinatior (Aech), Camponotus floridanus (Cf), Bombyx mori (Bm), Delia antiqua (Da), Acyrthosiphon pisum (Ap), Nurse cell (NC), oocyte (o). All embryo images are oriented with anterior to the left and dorsal side up.