Figure 3

Embryos resulting from dsRNA-mediated knockdown of Am-Toll , Am-Dpp , and Am-GB19066 . (A) DAPI stained wildtype (WT) embryo (stage 9, 48 h) with segments and appendages labelled. (B) Ventral view of a WT embryo at stage 9. (C) Stage 9 embryo following injection with dsRNA against Am-Toll. (D) A second example of typical stage 9 embryo after knockdown of Am-Toll, (E) Am-Dpp^RNAi, and (F) Am-Dpp dsRNA knockdown ventral side up. (G) and (H, ventral view) Am-GB19066^RNAi. All embryo images are oriented with anterior to the left and dorsal side up unless stated otherwise. Abbreviations: midline (ML), amnion (am), procephalic lobe (PC), mandible (mn), maxillae (mx), limb pair (lp), thorax (T), abdominal (A), terminal segment (T), tracheal pit (TP).