Figure 5
Expression of E. kanangrensis Wnt4. Anterior is to the left. (A) Ventral view. Red arrow marks the anterior border of expression. (B) Lateral view. (C) Embryo of (B), ventral view. Arrowhead indicates lack of expression in the posterior pit region. (D) Lateral view. (E) Anterior portion of embryo and close-up of jaw. Note that expression in the jaw is only in the mesoderm. (F) Ventrolateral view. Arrows indicate expression between the bases of the limbs. Asterisks mark expression in the ventral nervous system. (G) Embryo of (F). Close-up of slime-papillae-bearing segment. Arrowhead indicates staining in salivary gland. Asterisk marks expression in the ventral nervous system. (H) Embryo of (F). Close-up of head. Arrow and arrowhead indicate dot-like expression at base of frontal appendage. Note that expression in the frontal appendage is mesodermal. (A′), (G′) and (H′) represent DAPI staining of the embryos of (A), (G) and (H). fap, frontal appendage; hl, head lobe; j, jaw; L1, first walking-limb-bearing segment; m-a, mouth-anus furrow; pp, posterior pit region; sp, slime papilla; st, stage.