Figure 9

Expression of E. kanangrensis Wnt11. Anterior is to the left. (A) Ventral view. Arrow indicates strong expression in the posterior pit region. (B) Ventral view. Arrow indicates strong expression in the posterior pit region. (C) Lateral view. (D) Ventral view. Asterisks mark upcoming expression in segmental transverse stripes. (E) Embryo of (D), lateral view. Asterisks mark upcoming expression in segmental transverse stripes. (F) Close-up of frontal appendage. (G) Close-up of a jaw and a slime papilla. (H) Close-up of a walking limb. (I) Close-up of a slime-papillae-bearing segment, focused on the transverse stripe of expression. Note that this stripe is level with the opening of the salivary gland (arrowhead; cf. I′). (A′), (B′), (C′), (F′) and (I′) represent DAPI staining of the embryos of (A), (B), (C), (F) and (I). a, anus; fap, frontal appendage; hl, head lobe; j, jaw; L1, first walking-limb-bearing segment; pp, posterior pit region; sp, slime papilla; st, stage.