Developmental and light-entrained expression of melatonin and its relationship to the circadian clock in the sea anemone Nematostella vectensis

Table 2 Cosinor parameters of the clock genes where a significant curve fit was obtained

Parameter	Acrophase		Mesor		Amplitude
Gene	LD	Melatonin	LD	Melatonin	LD	Melatonin
NvTimeout	13.27 ± 0.32	0.04 ± 1.19	0.0079 ± 0.0008	0.0063 ± 0.0011	0.0049 ± 0.001	0.0042 ± 0.0015
NvCry1a	10.04 ± 0.174	6.24 ± 2.17	0.0066 ± 0.0003	0.0041 ± 0.0005	0.003 ± 0.0003	0.001 ± 0.0007
NvCry1b	13.18 ± 0.17	1.2 ± 1.18	0.011 ± 0.02	0.0144 ± 0.0023	0.007 ± 0.0008	0.0086 ± 0.0031
NvCry2	12.32 ± 1.13	1.11 ± 1.04	0.28 ± 0.033	0.30 ± 0.068	0.14 ± 0.044	0.343 ± 0.091

‘LD’ refers to animals kept in a light–dark cycle. ‘Melatonin’ refers to animals kept in a constant darkness cycle with melatonin (0.1 μM) supplementation. Values are mean ± SEM for five samples per assay. Bold indicates significant difference.

ISSN: 2041-9139