Figure 10

Expression patterns of Pdu-Hes5 and their schematic representations in Platynereis larvae and during posterior elongation (PE). Whole-mount in situ hybridization (WMISH) for the different stages indicated on each panel is shown. From 24 to 72 h post fertilization (hpf), Pdu-Hes5 is expressed in mesodermal lateral blocks. It is first restricted to the future segments at 48hpf, and then extends to the back of the embryos and the mesodermal segment addition zone (SAZ) at 72 hpf. The brain, stomodeum and the developing cone-shaped midgut cells also expressed Pdu-Hes5 respectively at 48 and 72 hpf. During PE, Pdu-Hes5 expression is found in both the mesodermal and ectodermal SAZ, and extended to the segment epidermis and in mesodermal stripes at the anterior part of segments. For the larvae, panels are ventral views (anterior is up), excepted for a 72-hpf picture and schema. Expressions patterns during PE are shown for both sides (anterior is up), V = ventral, D = dorsal.