Figure 8

Expression patterns of Pdu-Hes3 and their schematic representations in Platynereis larvae and during posterior elongation (PE). Whole-mount in situ hybridization (WMISH) for the different stages indicated on each panel is shown. In the 48 and 72 h post-fertilization (hpf) trochophore larvae, Pdu-Hes3 is expressed in the mesoderm of the (presumptive) parapodia. During PE, Pdu-Hes3 expression covers the exterior borders of the ventral nerve cord (VNC), with a more large and intense expression in the VNC of the segments recently produced by the segment addition zone (SAZ). Pdu-Hes3 is also expressed in ectodermal patches at the basis of the chaetae. Panels are ventral views (anterior is up) for the larvae and during PE.