Figure 2
From: Non-canonical Notch signaling represents an ancestral mechanism to regulate neural differentiation
Activation of Nvnotch suppresses NvashA expression. Images of animals stained for NvashA by in situ hybridization are shown (A-F). All images are lateral views with oral to the left. The relative focal plane is indicated to the left of each row of images. Animals with control wild-type Notch activity (A,D), with Notch activity reduced by injection of a Nvnotch morpholino (MO) (B,E), and with Notch activity overactivated by overexpression (OE) of the Nvnotch intracellular domain (Nvnicd) (C,F) are shown. (G) Quantitative (q)PCR analysis of the relative expression of NvashA is compared in animals with reduced Notch activity (DAPT, Nvnotch MO, Nvdelta MO) and increased Notch activity (Nvnicd OE), and to animals injected with a control MO. The red rectangle indicates a relative fold change of −1.5 to 1.5, which we consider to correspond with no change in expression level. (H) Quantification of the average number of NvashA-positive cells counted in the aboral domain (see Methods). N ≥20 animals counted for each treatment.