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Figure 4 | EvoDevo

Figure 4

From: Non-canonical Notch signaling represents an ancestral mechanism to regulate neural differentiation

Figure 4

Nvnotch suppresses neurogenesis by regulating NvashA expression. (A) Relative expression levels of NvashA target genes in animals with overexpressing (OE) Nvnicd (dark blue bars), overexpressing Nvnicd and NvashA (light blue bars), animals treated with DAPT (dark orange bars), and DAPT treated animals injected with the NvashA morpholino (MO) (light orange bars). Red rectangle represents relative fold change –1.5 to 1.5, which corresponds to no change in relative expression. Each treatment was repeated at least three times. (B-G) Aboral views of mRNA in situ images from two NvashA neural target genes are shown. Animals with overactive Nvnotch (B,E), control (C,F), and both overactive Nvnotch and overactive NvashA (D,G) are shown. Animals in (B-G) were quantified into phenotypic classes based on having no, weak, wild-type (WT)-like, or strong expression levels. The key is shown in the image and bars at the base of each image represent the percentage of animals in each phenotypic class.

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