Figure 7
From: Non-canonical Notch signaling represents an ancestral mechanism to regulate neural differentiation
Nvnotch does not regulate Nvhes expression in the Nematostella embryo. (A) Average relative fold change of Nvhes gene expression in animals injected with Nvnotch morpholino (MO; orange bars), treated with DAPT (blue bars), injected with Nvnicd:venus (dark purple bars), injected with Nvdelta:venus (light purple bars), or a control MO (grey bars. Red rectangle covers the region where the relative fold change ratio is equal to −1.5 to 1.5 and corresponds to no change in relative expression level. (B-E) Lateral views of late stage gastrula expressing Nvhes2 (B-C) or Nvhes3 (D-E). Oral is to the left. Deep focal plane is shown and superficial focal plane is shown in inset. We observed no discernable difference in Nvhes2 or Nvhes3 expression by in situ analysis between wild-type and Nvnotch MO injected animals. We scored N >80 embryos for each treatment. OE, overexpressing.