Figure 3

Expression of the nuclear receptor ultraspiracle (Usp) homolog Ppa-pnhr-1 is expressed prior to the first molt inside the egg. Expression is low in early stage 1 juvenile (J1) (16 hours) and high in late J1 (21 hours), indicative of a pending molt. The J2 developmental arrest serves as the negative control. Data represent eight total qPCR reactions from two independent cDNA samples at each time point. Each cDNA sample was collected from RNA from four pooled synchronized populations. The relative expression ratio of Ppa-pnhr-1 to Ppa-beta-tubulin was normalized to the no-reverse transcriptase negative control. Error bars represent standard error of the mean. **P ≤0.01 (Dunn’s test to J2 arrest).