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Figure 1 | EvoDevo

Figure 1

From: Evolutionary reconstruction of pattern formation in 98 Dictyostelium species reveals that cell-type specialization by lateral inhibition is a derived trait

Figure 1

Specificity of the prespore antibody. Phase contrast and fluorescence images of vegetative cells (A), cells from dissociated late culminants (B) and intact culminating (C) and mature fruiting bodies (D) of D. discoideum, which were stained with 1:2000 diluted antibodies, raised in rabbit against a mixture of D. discoideum and P. pallidum spores, and post stained with Fluorescein isothiocyanate (FITC)-conjugated donkey-anti-rabbit IgG. The spore antibodies react to spore walls (white arrows in B), and with vesicles in prespore cells, which presynthesize wall components (pink arrows in B). In multicellular structures stained prespore cells are separated from the unstained prestalk/upper cells (white arrows in C,D) by a well-defined boundary. The unstained rearguard cells (C, pink arrow) will form the lower cup and basal disc of the mature fruiting body (pink arrows in D). Scale bars, A/B: 10 μm; C/D: 100 μm.

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