Skip to main content

Table 1 Oligonucleotide primers used in this work

From: Evolutionary reconstruction of pattern formation in 98 Dictyostelium species reveals that cell-type specialization by lateral inhibition is a derived trait

Gene Primer name Restriction site Sequence
PPL_02670 PPL_02670F Xba I 5′-GATCTAGA GTTTGTTGATATTCATATGTTTC-3′
PPL_02670 PPL_02670R Bgl II 5′-CAAGATCT TTCCAGCATGAACCAATACAATTG-3′
PPL_10763 PPL_10763F Xba I 5′-AGCTCTAGA CACTAACACACCACCACTTAATCACAC-3′
PPL_10763 PPL_10763R Bgl II 5′-CACAGATCT TGGTCGTAGTGGTGGTTGCTC-3′
PPL_10763 PPL_10763_mutXbaF   5′-AACTAACTTCTCGAAATAAACTATACT-3′
PPL_10763 PPL_10763_mutXbaR   5′-AGTATAGTTTATTTCGAGAAGTTAGTT-3′
PPL_07208 PPL_07208F Xba I 5′-TCTAGA GGATGTATAATTATCTCATACTTCATCA-3′
PPL_07208 PPL_07208R Bgl II 5′-AGATCT CGAAATGGCTTTGGTAATATTA-3′
PPL_07586 PPL_07586F Xba I 5′-TCTAGA CCATTCGGATATCTAGTTTCCAAA-3′
PPL_07586 PPL_07586R Bgl II 5′-AGATCT GCTGATAATATAATATTGAATTTCAT-3′
PPL_04427 PPL_04427F Xba I 5′-TCTAGA ATAATCGAAATAAACAATATCAATA-3′
PPL_04427 PPL_04427R Bgl II 5′-AGATCT AATATTATTTAAAAAAATATTAGTTATTTCTTTAA-3′
  1. Restriction sites used for cloning are indicated in bold text.