Evolutionary reconstruction of pattern formation in 98 Dictyostelium species reveals that cell-type specialization by lateral inhibition is a derived trait

Table 1 Oligonucleotide primers used in this work

Gene	Primer name	Restriction site	Sequence
PPL_02670	PPL_02670F	Xba I	5′-GATCTAGA GTTTGTTGATATTCATATGTTTC-3′
PPL_02670	PPL_02670R	Bgl II	5′-CAAGATCT TTCCAGCATGAACCAATACAATTG-3′
PPL_10763	PPL_10763F	Xba I	5′-AGCTCTAGA CACTAACACACCACCACTTAATCACAC-3′
PPL_10763	PPL_10763R	Bgl II	5′-CACAGATCT TGGTCGTAGTGGTGGTTGCTC-3′
PPL_10763	PPL_10763_mutXbaF		5′-AACTAACTTCTCGAAATAAACTATACT-3′
PPL_10763	PPL_10763_mutXbaR		5′-AGTATAGTTTATTTCGAGAAGTTAGTT-3′
PPL_07208	PPL_07208F	Xba I	5′-TCTAGA GGATGTATAATTATCTCATACTTCATCA-3′
PPL_07208	PPL_07208R	Bgl II	5′-AGATCT CGAAATGGCTTTGGTAATATTA-3′
PPL_07586	PPL_07586F	Xba I	5′-TCTAGA CCATTCGGATATCTAGTTTCCAAA-3′
PPL_07586	PPL_07586R	Bgl II	5′-AGATCT GCTGATAATATAATATTGAATTTCAT-3′
PPL_04427	PPL_04427F	Xba I	5′-TCTAGA ATAATCGAAATAAACAATATCAATA-3′
PPL_04427	PPL_04427R	Bgl II	5′-AGATCT AATATTATTTAAAAAAATATTAGTTATTTCTTTAA-3′

ISSN: 2041-9139