Rp-cact expression and knockdown phenotypes. (A) Normalized mRNA levels for Rp-cact show small variability throughout embryonic development. (B) Rp-cact mRNAs in stage 1A embryos (0 to 6 hours) are mostly maternally provided, while gastrulation stage 2B (18 to 24 hours) mRNAs are generated zygotically, since levels of the former are unaltered, while the latter are decreased in unfertilized eggs (non fert). (C) Rp-cact is also expressed in the immune tissues of the midgut and fat body. (D) In immune tissues such as the gut, Rp-cact levels respond to activation of the Toll pathway using zymosan. (E,F) Ovaries resulting from control MalE (E) or Rp-cact (F) pRNAi show that egg chambers do not develop in the Rp-cact knockdown.