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Figure 4 | EvoDevo

Figure 4

From: A cleavage clock regulates features of lineage-specific differentiation in the development of a basal branching metazoan, the ctenophore Mnemiopsis leidyi

Figure 4

Nuclear divisions in cleavage-arrested embryos continue. Nuclei continue to divide after cytochalasin B treatment resulting in multiple nuclei in each cell, which later fuse into one or several large nuclei per cell. DNA staining with Hoechst (blue) merged with DIC images. (A) Embryo inhibited with cytochalasin B at the 2-cell stage. Multiple nuclei are visible along the periphery of the cells after 2 h of treatment. (B) A zygote arrested with cytochalasin B with one overly large nucleus. (C) Embryo inhibited with cytochalasin B at the 2-cell stage, with one large nucleus in the left cell and several large nuclei in the right cell. (D) Embryo inhibited with cytochalasin B at the 4-cell stage. Each cell has one large nucleus. Since all the embryos were inhibited prior to the 8-cell stage, none of them have differentiated comb cells.

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