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Table 1 Overview of the cytochalasin treatment experiments on Mnemiopsis leidyi embryos

From: A cleavage clock regulates features of lineage-specific differentiation in the development of a basal branching metazoan, the ctenophore Mnemiopsis leidyi

Developmental stage Treatment Observation Conclusion
8-cell stage Cytokinesis arrested with cytochalasin B 50% have combs All combs that were formed were labeled
16-cell stage 75% have combs
32-cell stage 83% have combs
8-cell stage Cytokinesis arrested with cytochalasin B, labeling of all four E blastomeres 62% (8/13) have combs that are labeled
38% (5/13) did not form combs
16-cell stage Cytokinesis arrested with cytochalasin B at 16-cell stage, labeling of all four E blastomeres at the 8-cell stage 95/128 (74%) formed combs that are labeled 99% of embryos that formed combs were labeled, E cell lineage forms combs
32/128 (25%) did not form combs
1/128 (0.8%) formed combs that are not labeled
16-cell stage Cytokinesis arrested with cytochalasin B at the 16-cell stage, labeling of all e1 micromeres at the 16-cell stage 27/36 (75%) formed combs that are labeled 100% of embryos that formed combs were labeled, e1 cells form combs
9/36 (25%) did not form combs
16-cell stage Cytokinesis arrested with cytochalasin B at the 16-cell stage, labeling of all 1E macromeres at the 16-cell stage No labeled combs Only e1 cells at 16-cell stage form combs and E1 cells do not form combs
8-cell stage Cytokinesis arrested with cytochalasin B at the 8-cell stage, labeling of all M blastomeres 19/27 (70%) formed combs that are not labeled 95% of the embryos formed combs that were not labeled and combs do not arise from M lineage cells
7/27 (26%) did not form combs
1/27 (4%) formed combs that are labeled
8-cell stage and 16-cell stage Cytokinesis arrested with cytochalasin B at the 8-cell stage or 16-cell stage, all E blastomeres were labeled Unlabeled cells have photoprotein M cells can make photoprotein even when they are cell-cycle arrested