Developmental stage | Treatment | Observation | Conclusion |
---|---|---|---|
8-cell stage | Cytokinesis arrested with cytochalasin B | 50% have combs | All combs that were formed were labeled |
16-cell stage | 75% have combs | ||
32-cell stage | 83% have combs | ||
8-cell stage | Cytokinesis arrested with cytochalasin B, labeling of all four E blastomeres | 62% (8/13) have combs that are labeled | |
38% (5/13) did not form combs | |||
16-cell stage | Cytokinesis arrested with cytochalasin B at 16-cell stage, labeling of all four E blastomeres at the 8-cell stage | 95/128 (74%) formed combs that are labeled | 99% of embryos that formed combs were labeled, E cell lineage forms combs |
32/128 (25%) did not form combs | |||
1/128 (0.8%) formed combs that are not labeled | |||
16-cell stage | Cytokinesis arrested with cytochalasin B at the 16-cell stage, labeling of all e1 micromeres at the 16-cell stage | 27/36 (75%) formed combs that are labeled | 100% of embryos that formed combs were labeled, e1 cells form combs |
9/36 (25%) did not form combs | |||
16-cell stage | Cytokinesis arrested with cytochalasin B at the 16-cell stage, labeling of all 1E macromeres at the 16-cell stage | No labeled combs | Only e1 cells at 16-cell stage form combs and E1 cells do not form combs |
8-cell stage | Cytokinesis arrested with cytochalasin B at the 8-cell stage, labeling of all M blastomeres | 19/27 (70%) formed combs that are not labeled | 95% of the embryos formed combs that were not labeled and combs do not arise from M lineage cells |
7/27 (26%) did not form combs | |||
1/27 (4%) formed combs that are labeled | |||
8-cell stage and 16-cell stage | Cytokinesis arrested with cytochalasin B at the 8-cell stage or 16-cell stage, all E blastomeres were labeled | Unlabeled cells have photoprotein | M cells can make photoprotein even when they are cell-cycle arrested |