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8-cell stage
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Cytokinesis arrested with cytochalasin B
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50% have combs
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All combs that were formed were labeled
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16-cell stage
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75% have combs
|
|
32-cell stage
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83% have combs
|
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8-cell stage
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Cytokinesis arrested with cytochalasin B, labeling of all four E blastomeres
|
62% (8/13) have combs that are labeled
|
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38% (5/13) did not form combs
|
|
16-cell stage
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Cytokinesis arrested with cytochalasin B at 16-cell stage, labeling of all four E blastomeres at the 8-cell stage
|
95/128 (74%) formed combs that are labeled
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99% of embryos that formed combs were labeled, E cell lineage forms combs
|
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32/128 (25%) did not form combs
|
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1/128 (0.8%) formed combs that are not labeled
|
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16-cell stage
|
Cytokinesis arrested with cytochalasin B at the 16-cell stage, labeling of all e1 micromeres at the 16-cell stage
|
27/36 (75%) formed combs that are labeled
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100% of embryos that formed combs were labeled, e1 cells form combs
|
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9/36 (25%) did not form combs
|
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16-cell stage
|
Cytokinesis arrested with cytochalasin B at the 16-cell stage, labeling of all 1E macromeres at the 16-cell stage
|
No labeled combs
|
Only e1 cells at 16-cell stage form combs and E1 cells do not form combs
|
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8-cell stage
|
Cytokinesis arrested with cytochalasin B at the 8-cell stage, labeling of all M blastomeres
|
19/27 (70%) formed combs that are not labeled
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95% of the embryos formed combs that were not labeled and combs do not arise from M lineage cells
|
|
7/27 (26%) did not form combs
|
|
1/27 (4%) formed combs that are labeled
|
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8-cell stage and 16-cell stage
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Cytokinesis arrested with cytochalasin B at the 8-cell stage or 16-cell stage, all E blastomeres were labeled
|
Unlabeled cells have photoprotein
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M cells can make photoprotein even when they are cell-cycle arrested
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