Figure 5

Craniofacial expression pattern of selected members of the coexpression network. (A) Whole-mount in situ hybridization for Ctsk, Mmp2, Ogn, Sfrp1 and Sparc at stage 3 in the aquaculture (AC) morph, ventral and lateral views. Expression of all genes can be seen anterior to and surrounding the mouth (white arrows), as well as in the pharyngeal arches. Overlapping expression is highly pronounced in the perichondrial region of the lower jaw (red arrows and dashed squares). (B) Comparison of Timp2 expression between the four morphs at stage 1. An overall weaker staining is obtained for Timp2 in AC compared with the other morphs. Pronounced expression of Timp2 can be seen in the frontonasal region (black arrows) and at the pharyngeal arches (green arrows). (C) At stage 5, both AC and the planktivorous (PL) morph show weaker expression of Timp2 in an area anterior to the mouth (white arrows). A clear difference between the benthic and limnetic morphs can also be seen in the expression pattern and levels of Timp2 in the pharyngeal arch region (red and white dashed squares highlight some of the differences). LB, Large benthivorous morph; SB, Small benthivorous morph. Scale bar = 1 mM.