Alignment of deduced amino acid sequences of Ino-POU2, Ino-POU3, Ino-POU4, and Ino-POU6 compared to selected bilaterian orthologs. Residues colored in black are identical, dark gray-colored ones are 80 to 100% conserved and white ones less than 60% conserved. The POU-domain comprises an N-terminal POU specific domain (red) and a C-terminal POU-type homeodomain (blue), which are connected via a linker region (green). The 36 amino acids of the sequence Hs-POU6-2 have been deleted, as indicated by an arrowhead. GenBank accession numbers of amino acid sequences used for alignment: I. notoides: Ino-POU2: [GenBank:KM892881], Ino-POU3: [GenBank:KM892882], Ino-POU4: [GenBank:KM892883], Ino-POU6: [GenBank:KM892884]; Branchiostoma floridae: Bf- POU1: ABP01321.1, Bf- POU2: XP_002610677.1, Bf-POU3: AAL85498.1, Bf- POU4: ABC42926.1, Bf- POU6: XP_002608796.1; Capitella teleta: Cap-POU2: ELU09478.1, Cap-POU3: ELU03597.1, Cap-POU4: ELT93355.1, Cap-POU6: ELU13951.1, Homo sapiens: Hs-POU2 isoform 1: NP_002688.3, Hs-POU2 isoform 2: NP_002689.1, Hs-POU2 isoform 3: NP_055167.2, Hs-POU3 isoform 1: NP_002690.3, Hs-POU3 isoform 2: NP_005595.2, Hs-POU3 isoform 3:, NP_006227.1, Hs-POU3 isoform 4:, NP_000298.3, Hs-POU4 isoform 1: NP_006228.3, Hs-POU4 isoform 2: NP_004566.2, Hs-POU4 isoform 3: NP_002691.1, Hs-POU6 isoform 1: NP_002693.3, Hs-POU6 isoform 2: NP_009183.3.