Figure 6

Histological sections of hermaphroditic and dioecious Lepidium species. Floral developmental stages noted below follow Arabidopsis thaliana stages [20, 61, 62]. (A-C) Bright field images of flowers at the pre-meiotic stage of microsporogenesis, stage 9, area outlined in red magnified below; from left to right, (A) hermaphroditic L. tenuicaule, (B) staminate L. sisymbrioides, and (C) carpellate L. sisymbrioides. (D, F, H) Bright field images of anther from respective flower above. (E, G, I) Bright field images of ovule from respective flower above. (J, L, N) Confocal microscopy of anther from respective flower above. (K, M, O) Confocal microscopy of ovule from respective flower above. (P-R) Bright field images of flowers at the microgametogenesis stage, stages 11 to 13, area outlined in red magnified below; from left to right, (P) hermaphroditic L. tenuicaule, (Q) staminate L. sisymbrioides, and (R) carpellate L. sisymbrioides. (S, W) Bright field images of anther from respective flower above. (U) Bright field images of ovule from male flower of L. sisymbrioides at stage 13 from Additional file 2. (T, V) Bright field images of ovule from respective flower above. (X) Bright field images of anther from female flower of L. sisymbrioides at stage 12 from Additional file 2. (Y, Z2, Z4) Confocal microscopy of anther from respective flower above. (Z1, Z3, Z5) Confocal microscopy of ovule from respective flower above. (A-T, V-W, Y-Z5) Stained with Safranin O and Fast Green FCF, (U, X) stained with Toluidine Blue O. Scale bar = 100 μm. E, endothecium; ES, embryo sac; F, funiculus; G, gynoecium; I, integuments; M, microspores; N, nucleus; O, ovule; P, pollen; PMC, pollen mother cells; Se, sepal; Sg, stigmatic papillae; St, stamen; Sy, style; T, tapetum; V, vacuolated cell.