Figure 1

Immunolabeling of larvae of Wirenia argentea. Maximum intensity projections of confocal image stacks; apical is up and scale bar equals 40 μm in all panels. A: Labeling of acetylated α-tubulin-like immunoreactive (−LIR) components; 0 to 1 days posthatching (dph) larva showing developing apical organ (arrowheads) and abapical neurogenic domain (asterisk) with outgrowing neurite bundle (arrow). B: Labeling of acetylated α-tubulin-LIR components; 1 to 2 dph larva showing abapical neurogenic domain (asterisk) with outgrowing neurite bundle (arrow) as well as a second neurite bundle (double arrowhead) growing out from the neuropil of the apical organ. C: Labeling of acetylated α-tubulin-LIR components; 1 to 2 dph larva showing developing apical organ (arrowheads) and abapical neurogenic domain (asterisk) with a pair of outgrowing neurite bundles (arrows). D: Labeling of acetylated α-tubulin-LIR (blue) and FMRF-amide-LIR (yellow) components; 2 to 3 dph larva showing abapical neurogenic domain (asterisk) with a pair of outgrowing neurite bundles (arrows). Abbreviations: ao, apical organ; at, apical tuft; np, neuropil of apical organ; pt, prototroch.