Figure 6

Immunolabeling of a larva of Wirenia argentea. Maximum intensity projection of confocal image stack; apical part upwards; scale bar equals 20 μm. Labeling of acetylated α-tubulin-like immunoreactive (−LIR) (blue) and serotonin-LIR (orange) components; 15 to 16 days posthatching larva, detail of apical organ region scanned in right lateral aspect showing the cerebral commissure (arrow) with the originating longitudinal neurite bundles (arrowheads) and the flask-shaped cells of the apical organ with their projections (double arrowheads) to the surface; note that two of the apical organ cells are serotonin-LIR (asterisks). Abbreviations: at, apical tuft; pnp, prototrochal nerve plexus.